Ohio Administrative Code|Rule 901:1-5-12 | Brucella canis.

                                                
(A) Brucella canis tests.
(1) As used in this rule,
			 a brucella canis test means one of the following:
(a) An indirect fluorescent antibody test (IFA
				test);
(b) A tube agglutination test (TAT test); or
(c) A brucella canis culture test.
(2) The tests contained
			 in paragraph (A)(1) of this rule may only be conducted by a laboratory approved
			 by or operated by the department of agriculture, except that a laboratory not
			 approved or operated by the Ohio department of agriculture may conduct an IFA
			 test.
(3) Laboratories approved
			 or operated by the Ohio department of agriculture may combine no more than five
			 samples for IFA testing. If the composite sample is positive for brucella
			 canis, each canine sample making up the composite sample must be tested
			 individually.
(4) Any blood samples
			 which are taken to conduct a brucella canis test shall only be taken by an
			 accredited veterinarian, or by a registered veterinary technician who is
			 supervised by an accredited veterinarian.
(B) Brucella canis
		  classifications.
(1) Suspect.
(a) A canine shall be classified as brucella canis suspect, if it
				tests positive for brucella canis using the following tests:
(i) An IFA test conducted by a laboratory; or
(ii) A TAT test, at dilutions of 1/50 or 1/100.
(b) A canine test which has been classified as brucella canis
				suspect according to paragraph (B)(1)(a) of this rule, shall
				either:
(i) Be referred to the
				  Ohio department of agriculture for confirmation of its brucella canis status;
				  or
(ii) Be humanely
				  euthanized.
(2) Negative.
A canine shall be classified as brucella canis
			 negative if, through tests conducted by the Ohio department of agriculture or
			 an approved laboratory, the canine tests negative for brucella canis using the
			 following tests:
(a) An IFA test; or
(b) A TAT test
(3) Positive.
A canine shall be classified as brucella canis
			 positive if, through tests conducted by the Ohio department of agriculture or
			 an approved laboratory, the canine tests positive for brucella canis using the
			 following tests:
(a) A TAT test, at dilutions 1/200; or
(b) A brucella canis culture test.
(4) Reclassification.
Brucella canis positive animals may be
			 reclassified by the Ohio department of agriculture to brucella canis suspect
			 pending the following:
(a) The animal's history;
(b) Clinical signs of the animal;
(c) Vaccination history;
(d) An epidemiological study of the animal;
(e) Examination; and
(f) Further testing.
(C) Identification and
		  reporting.
(1) All accredited
			 veterinarians who conduct a brucella canis test, submits a sample for brucella
			 canis testing, or employs any other method of detection for brucella canis,
			 shall include in the report as required in division (C) of section 941.24 of
			 the Revised Code, on a form approved by the Ohio department of agriculture, the
			 following information:
(a) Name and address of the canine's owner;
(b) Date of test or sample collection;
(c) Identification, age, sex, and breed of each individual animal
				tested or sampled; and,
(d) The results of the test performed, if available.
(2) All accredited
			 veterinarians and laboratories who conduct brucella canis testing shall report
			 the results of the tests to the Ohio department of agriculture within seven
			 days on a form approved by the Ohio department of agriculture.
(3) An accredited
			 veterinarian must identify any canine to be tested for brucella canis with any
			 of the following items which have been approved by the Ohio department of
			 agriculture:
(a) A microchip;
(b) A tattoo;
(c) A tamper proof eartag; or
(d) A neck chain with an individual official USDA identification
				tag may only be used by USDA licensed kennels.
(D) Quarantine.
(1) Pursuant to Chapter
			 941. of the Revised Code, the Ohio department of agriculture shall quarantine
			 any canine on any premises that has been exposed to or diagnosed as brucella
			 canis suspect or brucella canis positive animal.
(2) A brucella canis
			 suspect canine shall be released from quarantine upon two negative tests at
			 least sixty days apart that were conducted by the Ohio department of
			 agriculture or an approved laboratory.
(3) A brucella canis
			 positive canine on an Ohio department of agriculture licensed kennel premises
			 shall be released from quarantine upon either:
(a) The animal has been humanely euthanized; or
(b) All of the following have been met:
(i) Ovario-hysterectomy
				  or castration;
(ii) The owner has been
				  advised as to the zoonotic potential; and
(iii) The canine cannot
				  be offered for sale, trade, barter, or otherwise cannot leave the premises
				  without prior permission of the Ohio department of agriculture.
(4) A brucella canis
			 positive canine not on an Ohio department of agriculture licensed kennel
			 premises shall be released from quarantine upon the following conditions being
			 met:
(a) Ovario-hysterectomy or castration;
(b) An epidemiological study conducted by the Ohio department of
				agriculture;
(c) Two negative tests at least sixty days apart that were
				conducted by the Ohio department of agriculture or an approved
				laboratory;
(d) The owner has been advised as to the zoonotic potential;
				and,
(e) The canine cannot be offered for sale, trade, barter, or
				otherwise cannot leave the premises without prior permission of the Ohio
				department of agriculture.
(E) Selling, movement, and disposal of
		  animals.
(1) No canine known to be
			 brucella canis suspect or brucella canis positive shall be offered for sale,
			 trade, barter, or otherwise removed from the premises.
(2) Puppies born to a
			 brucella canis suspect dam shall not be offered for sale, trade, barter, or be
			 removed from the premises unless the dam is later classified as brucella canis
			 negative. These puppies may be isolated and quarantined in accordance with
			 paragraph (D) of rule 901:1-5-12 of the Administrative Code and shall be tested
			 after they reach six months of age and must be re-tested no sooner than sixty
			 days after the initial test.
(3) Puppies born to a
			 brucella positive dam must not be offered for sale, barter, or otherwise
			 removed from the premises without prior permission of the department. These
			 puppies may be isolated and quarantined in accordance with paragraph (D) of
			 rule 901:1-5-12 of the Administrative Code and shall be tested after six months
			 of age and must be re-tested no sooner than sixty days after the initial
			 test.
(4) Puppies under six-months of age which
			 are classified as brucella canis positive may be retested after six-months of
			 age and must be re-tested no sooner than sixty days after the initial
			 test.